FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698174
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21900.016.8265131
GTCCTAG34650.014.9520931
GTTCTAG47850.014.258591
GTGCTAG38050.014.1913581
CGAGACT57100.014.1850884
GCGAGAC58500.014.1573433
GTATTAG40750.013.6092081
GAGCGAG62350.013.10775851
GTCTTAG37100.012.7845761
GTACAGG45400.012.6974961
AGCGAGA67500.012.26962
GTATAGG25750.012.1853531
ACGACTC16650.012.060245573
GCGCTAG4558.303687E-912.0280921
GTCCTAA35550.012.00779151
GGTCTAG26450.012.0008091
GTGCAGG80050.011.9414431
GGTCTAA29750.011.7733791
GTACTAA35550.011.69991
CTAGACT37200.011.47514254
Produced by FastQC (version 0.11.5)