FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12515790
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG38250.016.0221561
GTGCTAG38150.015.7772941
GTTCTAG46900.015.3227431
GTACTAG21850.015.1926351
GTATTAG39700.013.5073451
GTCTTAG36850.013.4630831
GCGAGAC55750.013.3490163
GGTCTAG26700.013.2526771
CGACGAC16050.013.19568172
CGAGACT57100.012.9060954
GAGCGAG57850.012.863821
GTACAGG43700.012.8553061
GTACTAA33500.012.6315411
GTGTTAG45700.012.532181
ACGACTC15850.012.44065873
GTGCTGG192250.012.2387341
GTCCTAA34000.012.23121
GTCTACG7000.011.9859621
GTGCAGA75050.011.8113271
AGCGAGA61900.011.7276912
Produced by FastQC (version 0.11.5)