FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12515790
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG35400.014.7477521
CGAGACT54250.014.2635224
GCGAGAC54500.013.9301493
GTATTAG39000.013.6672551
GTTCTAG43850.013.6542331
CGAGACG15300.013.59878573
GTACTAG21500.013.41472151
GTCTTAG37300.013.409261
GCCCTAG29550.012.9725361
GTCCAGG87100.012.4069961
GAGCGAG60000.012.35201551
GTGCTAG39050.012.3408751
GGTCTAG24400.012.1195981
GTGTTAG47350.012.1052211
GTCCTAA33250.012.0779741
TCAGGAC58450.012.0520733
AGCGAGA62850.011.7312
CAGGACC65350.011.7290924
CCAGGAC85550.011.64746953
GTATAGG23050.011.5623211
Produced by FastQC (version 0.11.5)