FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11412958
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC53550.015.541253
GTCCTAG32700.015.0733191
CGAGACT54150.013.7512534
GAGCGAG57800.013.707391
GTACTAG20200.013.5560381
GTGCTAG35700.013.0907541
GTATTAG38200.012.9986541
AGCGAGA60700.012.928752
GTGTTAG43450.012.8565521
GTACTAA33300.012.7185221
GTTCTAG41400.012.5230711
GTACAGG42450.012.2993231
TCGGGAC9550.012.23086553
GTGCAGA69300.012.170311
GTACGGG6051.8189894E-1212.0697281
GCTCTAG29300.011.9626251
GTGCAGG75150.011.9516811
GTCCTAT24750.011.8015121
CAGGACT64500.011.6578414
CCAGGAC76650.011.57193
Produced by FastQC (version 0.11.5)