FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11412958
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20000.018.069551
CGAGACT48050.016.2539794
GCGAGAC47750.016.2031673
GTCCTAG29050.016.0844381
GAGCGAG54200.013.8742661
AGCGAGA56650.013.2718482
GTATTAG38500.013.1794171
GCTCTAG30850.013.0160631
GTTCTAG39350.012.5236561
GTGCTAG34950.012.4291631
GTCTTAG30700.012.3662221
GTACTAA31950.012.1109181
GGTCTAG22800.012.007941
GTACTAC17700.011.9618081
GCCCTAG26700.011.4844481
GTCCAGG74750.011.4762171
GTGCTGG177650.011.4248811
GTACAGG41400.011.28630351
GGTCTAA26900.011.2633581
CTAGACT31850.011.2293834
Produced by FastQC (version 0.11.5)