FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009632
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC52200.015.4529693
GTACTAG19850.015.0840881
CGAGACT51800.014.7266754
GGTCTAG23400.014.5121841
GTGCTAG35200.014.1078861
GTCCTAG30750.013.89333251
GTCTTAG33200.013.6379581
GAGCGAG56000.013.5625391
GCTAGAC23000.013.4890463
GTATTAG34150.013.4724181
AGCGAGA59750.012.645112
GTCCTAA29550.012.48043251
GTGTTAG43800.012.171511
GTTCTAG40500.012.081351
GTCTAGC23250.011.9359321
CTAGACG4901.9408617E-911.9182394
CGAGACG13800.011.90182973
GTGCAGG75800.011.8503621
GTCTAAG26500.011.8499981
CTAGACT32450.011.810354
Produced by FastQC (version 0.11.5)