FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009632
Sequences flagged as poor quality0
Sequence length79
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18700.015.6146841
CGAGACT49600.015.1577534
GCGAGAC49850.015.08194353
GTTCTAG38600.014.6564641
GTCCTAG31200.014.2721721
GTGCTAG34000.014.1703241
GAGCGAG56400.013.0077381
GTATTAG36300.012.8702851
CGACGAC14300.012.76466872
AGCGAGA59900.012.1253782
GTACAGG39900.012.0749651
GTCCTAC19100.012.0390441
GGTCTAG22400.011.8948691
GTGCTGG163750.011.880391
GTCTAGG28500.011.654171
CCGACGA15400.011.61568971
GTACTAA29700.011.5519741
CTATACT37050.011.4266554
CAAGACT89800.011.3797044
GTGCAGA66150.011.3663811
Produced by FastQC (version 0.11.5)