FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709798
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA12250.016.5844751
GTATTAG7700.016.0989511
GTCCTAA8200.015.9571591
GTCCTAG9350.015.83589651
GTACTAA7050.015.1411331
GTACAGG14400.014.5865681
GTATTAA10400.014.237081
TCTAGAC8250.013.7737323
GTATAAG7100.013.5795541
CTATACT7200.013.3911284
GTACTAC5450.013.2680951
GCTCTAG10500.013.1176631
GTGCGTA2103.047052E-413.1176631
GTACTAG3952.39379E-913.0761521
TCTAGCG1850.001751585213.0292063
GTATAGA8300.012.8608431
CTAACCG2153.7167326E-412.8127744
TCTATAC7800.012.8025083
GTTCTAA10800.012.7532841
CAATACG2457.871275E-512.6493464
Produced by FastQC (version 0.11.5)