FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766558
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG9100.019.3041361
ATCGTAC1503.5701218E-416.0748043
GTACTAG3950.015.6962911
GTATTAA11100.015.5156031
GTTCTAA8750.014.9588141
TAGTGCG1656.430208E-414.87715657
TTAGCGA1400.004180836514.76022410
TTGCGCG1657.372723E-414.61113112
TAGGACG2657.232593E-714.2983444
GTCTTAA10200.014.1830861
CTACTAG5750.013.7778551
GTACTAA7500.013.7778541
CGAATCG1500.00660753813.7762089
CTAGACT7100.013.5843414
GTATATA11350.013.3529861
CCTATCG1550.00820807813.3318149
CTTAGAC6500.013.2484653
GTACGGA2609.3240815E-613.2479361
GTATTAG7550.013.2303911
TAGACAG14600.013.2121685
Produced by FastQC (version 0.11.5)