FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686792
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG8150.018.5906641
GTATTAG6450.017.6178991
TACTAGC4100.015.9576752
GTATTAA9700.015.6199921
GTATAGG5250.015.0858011
GTACTAG5300.014.9434831
GTGCTAA8850.014.0074511
GCGCTAT1500.0066145413.7739941
GTCCTAA7350.013.5865921
CGAATCG1550.00812876313.35140445
GTCTTAG7550.013.2266821
GTGCTAG8650.013.1370461
GTACAGG15850.013.0353241
GATCTAA6650.012.9454821
CTATACT7800.012.8030984
CTAACGT1900.002139761312.6869174
GTATAAG6250.012.6720731
CTAGGGT7900.012.6410334
GGTCTAG6000.012.6261621
TATAGAC6550.012.6173983
Produced by FastQC (version 0.11.5)