FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686792
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG10150.016.6348971
GTGCTAG9000.016.4632681
GTATTAG6550.016.3083691
GTTCTAG9850.015.7422361
GGTCTAA6350.015.7367271
GTCCTAG9450.015.6793031
GTCTTAG7600.015.4154231
CCCCTAA8050.014.9817421
GTACTAA6650.014.5086341
TAGTGCG1500.005852144714.030972557
GCTCTAG10700.013.84760951
GTCCTAA8000.013.7832011
CTATCCG1500.00662033513.7721729
ACTATAC5100.013.5045113
GTCGTAC2609.286716E-613.2530781
TCTATAC6600.013.044133
GTACTAC5550.013.0381641
GTCTAGA8200.013.0268061
GTGCTAA9050.012.9455481
GTGTTAA10400.012.9217511
Produced by FastQC (version 0.11.5)