FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9447291
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG47650.027.691046145
TCTCGTT38950.021.405298145
TGCCGTG58000.020.749659145
GTATTAG30900.019.9474721
GTCCTAG39000.019.7091871
GTATACG6900.018.9169371
CTAGACT28050.018.8698794
GTATTAA46700.018.6333871
CGTCTTA29500.018.4319145
GTACTAG18100.018.4292071
GTCTTAG34650.018.4164891
GTGCTAG34050.018.3150751
GCCCTAG35400.018.2311531
GTTCTAG41450.018.019391
GGTCTAA30250.017.9789071
GTGCTAA37550.017.5735241
GCTCTAG40650.017.3036841
TCTCCGC102350.017.21271145
CACGAGC65000.017.17664145
CTTAACG9000.016.9184473
Produced by FastQC (version 0.11.5)