FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9447291
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC120900.036.38289145
CGCCGTT16900.031.301922145
GTACTAG17750.024.544951
TTATACC75200.022.356562145
GTCCTAG35750.020.717311
TTACGTT22450.020.335697145
GTCTTAG32900.020.3049161
CCGTATA15050.020.223095145
GTATTAG28350.019.9779431
CTGCCGC217650.019.510742145
GTTCTAG45250.019.4167271
CGTGTAT21050.019.27839145
GCCGACC37350.019.01386145
GTACTAT27950.018.964891
GTACTAA27250.018.9191271
GACGACA38550.018.60997145
GTGCTAG34900.018.3090781
CGAATCG6453.6379788E-1217.9767519
GTCTTAA39800.017.8793721
GTATTAA47550.017.8667091
Produced by FastQC (version 0.11.5)