FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9458540
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT17200.033.279808145
CCGACGC125150.033.116726145
CCGTATA13550.031.014938145
GTATACG7150.022.3355311
TTATACC77250.022.042028145
TAGATCG11800.020.263483145
TTACGTT21900.019.851316145
GTCCTAA32500.019.6552661
CTGCCGC217300.019.47311145
GTTCTAG44300.019.3356111
GCCGACC37350.019.205551145
GTTCTAA44700.018.8377971
GTATTAG32200.018.7112081
GTCCTAG39350.018.4473881
GACGACA38300.018.161621145
GTGCTAG36850.017.9260061
GCTCTAG41500.017.8415131
GTACTAG16800.017.7155321
CGTGTAT23750.017.694838145
GTACTAA26450.017.2899781
Produced by FastQC (version 0.11.5)