FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304249
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36650.026.1176151
CGTCTTG60350.023.546099145
GTACTAA26500.020.523381
TGCCGTG66650.020.341436145
GTGCTAG33700.020.011821
GTACTAG17750.019.6099471
GTTCTAG38550.019.5633161
GTATTAA44500.019.2289491
GTATTAG28800.019.1361521
TCTAGAC32700.018.6246453
GTCTAGA32600.018.462651
GGTCTAG27900.018.1939661
GTATAGG22450.018.088611
GTACTAT29350.017.2951151
GTTCTAA43750.017.2380751
CTATACG6505.820766E-1116.7314824
GTACAGA73700.016.7268811
GTCCTAA32000.016.5427021
CTATACT31150.016.5255834
GTATATA51350.016.5226211
Produced by FastQC (version 0.11.5)