FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9304249
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT20750.032.142036145
TTACGTT26700.025.79384145
GTACTAG17550.023.5574321
CCGACGC196750.022.254911145
GTTCTAG41400.021.8997861
CCGTATA19550.021.878065145
GTCCTAG35450.021.483411
GCCGACC41000.020.864204145
TAGATCG17450.019.941116145
GTCTTAG34200.019.2994731
GTATTAA45800.018.8456731
GGTCTAA29300.018.5662351
GACGACA43400.018.207085145
GTACTAA28150.018.0363981
TAGACAG50600.017.9067385
GTCCTAA30000.017.6494731
GTATAGG23450.017.6304021
TTATACC104500.017.620615145
ACGTGTT32300.017.506357145
CTGTACT72500.017.2966794
Produced by FastQC (version 0.11.5)