FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9344375
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT21700.032.403534145
GCCGACC43250.024.470741145
CCGTATA20700.023.463068145
GTACTAG17400.022.089981
CCGACGC212350.021.642996145
GTATTAG28050.021.2006741
GACGACA43450.021.021378145
GTTCTAG42200.020.4504661
GTCCTAA31300.020.1578241
TTACGTT30650.019.86686145
TTATACC113550.019.662737145
GTACTAA27700.019.6358721
CGTGTAT26000.018.959023145
GTCCTAG36000.018.9362531
CCTATAC23600.018.4312363
GTGCTAG35900.017.9789471
TCTAGAC31100.017.7161143
TAGATCG17650.017.660545145
CTATACT32500.017.6220594
GTATAGG23500.017.5903991
Produced by FastQC (version 0.11.5)