FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9820739
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA70000.029.332216145
GGGGGGG639300.019.755838145
TATGCCG29650.018.520283145
GTACTAG19450.017.8220391
GTCCTAG36900.016.5960671
GTGCTAG41750.015.6367921
GCTCTAG45650.015.060241
GTCCTAA34300.014.8222551
GTATTAA50850.014.4290751
CCTGTCG30303.6379788E-1214.183204145
TCTAGAC33550.013.9483433
GTACTAA30350.013.7056461
GTATAGA36650.013.3989471
GTCTTAG37850.013.1267821
GTGCTAA37100.013.0807041
GTATTAG36450.012.9969671
GTATAAG33400.012.9730031
GTCTAGA35200.012.8019941
GTTCTAA48850.012.7727631
GTACTGG65350.012.6420111
Produced by FastQC (version 0.11.5)