FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9820739
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA68650.021.387997145
GTCCTAG39700.018.9726471
CCGTATA11452.0305924E-618.614697145
GTATTAG33850.017.1165561
GTTCTAG45600.017.0260961
CCTGTCG31250.016.67215145
GTCTTAG35250.015.9436491
GTCCTAA35200.014.8140891
GTACTAA31850.014.7350181
TAGCACG11508.165147E-414.415149145
GTATACG8200.014.13159751
GCCCTAG39300.013.7108851
GTATTAA49250.013.6466741
CTAGACT34400.013.2862334
GTACATA45650.013.0728871
CTATACT35250.012.9658564
GGTCTAA30650.012.854451
GTACTAT31150.012.8341221
GCTCTAG46500.012.7093191
GTGCTAG37850.012.705371
Produced by FastQC (version 0.11.5)