FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9861614
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA69500.024.781979145
CCTGTCG30600.021.5891145
GTACTAG18700.018.9014191
CGACGAA16355.7843863E-1018.759598145
GTCCTAA33550.016.2345981
GTACTAA30300.015.6811321
GTATTAG34050.015.3155151
GTCCTAG40350.015.22188951
GCTCTAG43900.015.0468831
GTATTAA48100.014.81720351
GCCCTAG38600.014.7110941
GCCTTAG39300.014.4490631
TATACTG44250.014.2535225
GTCTTAG37050.014.0753931
GTTCTAA44950.014.0508631
GTTCTAG45850.013.9014321
GTGCTAG40050.013.7444471
TGACGCG13802.1385163E-413.677566145
GTATAGG27200.013.6338111
GTCCTAT34500.013.6041721
Produced by FastQC (version 0.11.5)