FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667058
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA80450.032.616367145
GGGGGGG613250.021.431969145
TATGCCG42500.018.576899145
GTCCTAG37650.016.778361
CCTGTCG36000.016.12578145
GTGCTAG38350.014.8097841
GTCTTAG37600.014.6427891
GTATTAG31900.014.5340741
GTTCTAG45450.014.1539331
GTACTAG19650.013.8619181
GTATAAG33900.013.8475641
GTACTAA29050.013.7654691
GTATTAA48950.013.6154871
GTACTAT28600.013.3741441
GTCCTAT30850.013.3380161
GCCTTAG37200.013.2423191
CGCGTGT10550.00873611713.2063265145
CTATACT35950.012.8973834
GTTCTAA46400.012.8649251
GCTCTAG43750.012.7168981
Produced by FastQC (version 0.11.5)