FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667058
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG38700.026.846987145
CGACGAA16500.023.788055145
CTGTCTA71900.020.230453145
GTCCTAG37200.018.0788671
TGACGCG15901.08721E-817.425213145
GTACTAG21200.016.4086021
CTAGTAC19300.015.9124313
GCCGTAT26250.015.832051145
GTTCTAG43300.015.6658571
CGCCGTA26750.015.536125145
CCGGTCG15001.96976E-615.392272145
CGAATCG8900.014.974629
GTCGCTT28001.8189894E-1214.842547145
GTCTTAG37000.014.7293071
GGTCTAG31900.014.17620851
GTATAGG25400.014.1518811
GTACTAA29100.014.1456061
GTATTAG32050.014.1098611
GCTCTAG41700.014.0424061
CTATACT35300.013.95297054
Produced by FastQC (version 0.11.5)