FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614904
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA82000.037.59434145
GGGGGGG572400.021.582722145
TCGTATG43550.017.432405145
TCGCGTT8600.002392265216.050344145
GGTCTAA28200.015.6320311
GTGCTAG39650.015.50649451
CTATACT34800.015.50106054
GTCTTAG34750.015.3562071
GTTCTAG41750.015.2822441
GTACTAG17900.015.2298541
GTCCTAG37700.015.077721
GCTCTAG44900.014.4684711
GTCCTAA36750.014.3626631
GGTCTAG32850.014.1255551
GTATTAA52050.013.81821251
GCCCTAG38450.012.6716711
TCTATAC33500.012.6394743
TATACTG44550.012.6295695
TATGCCG41950.012.613261145
GTACTAA28100.012.3849961
Produced by FastQC (version 0.11.5)