FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614904
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA80050.022.994743145
CCTGTCG37250.021.001606145
CCGTATA9208.229234E-620.007925145
CGACGAA17502.0027073E-917.092484145
TGACGCG15201.2839337E-716.651333145
GTTCTAG44950.016.0160831
GCCCTAG35950.015.3422491
GTTCTAA45300.014.9951891
GTACTAG18600.014.9827861
GTCCTAA32800.014.8685891
TAGCTCG10906.768715E-414.776495145
GTCTTAG35250.014.4939871
GTATTAG32050.014.491931
GTCCTAG38950.014.4586771
GTACTAA28300.014.3607091
GTCTCGT34350.013.396863145
GTACAGG65450.013.2172451
GGTCTAA30000.013.1598811
CCGACGG19402.0286043E-613.046405145
CTAAGCG11150.013.0118014
Produced by FastQC (version 0.11.5)