FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10425287
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA72250.038.590084145
GGGGGGG571850.016.661629145
GTCCTAG38550.016.2820361
GTCTTAG40100.015.9480161
GTATTAG35550.015.8238171
GTTCTAA49700.015.01211
GTACTAG19350.014.9948591
GTATATA62500.014.7799121
GTTCTAG46050.014.6589361
GTGCTAG40900.014.6226241
GTACTAA31500.014.2866461
TATACTG51800.013.7178175
GTACTAT34150.013.524811
GTATATG50850.013.50810051
GTGTAGG51950.013.3360611
CTATACT36000.013.3233654
GTGTATA48600.013.2805931
CCCTTAG43550.013.1889691
GTATAGG26050.013.184011
GTACCGT11250.013.1595746
Produced by FastQC (version 0.11.5)