FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10425287
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA72050.024.437798145
CCTGTCG31350.021.061523145
GTCCTAG41200.020.7317681
GTATTAG33550.018.563841
GTCTTAG37600.018.1418291
ACGCGTT7300.001074273518.089828145
GTACTAA32100.017.1849781
GTACTAG20450.016.5330331
GTCCTAA34950.015.7836281
CGACGAA15502.6581256E-715.619497145
GTATATA58850.015.42112351
GTGCTAG42700.015.0026131
CCTATAC26200.014.7117513
GTATAGG29050.014.4971711
GTTCTAA50550.014.3156061
GTCTTAA46350.014.2051011
GCTCTAG50500.014.0948661
GTGTTAG41050.013.8716821
CTATACT37300.013.6723124
GGTCTAA32050.013.5103281
Produced by FastQC (version 0.11.5)