FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457178
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT6657.048093E-726.549604145
CTGTCTA74500.021.62501145
GACGATA15754.0017767E-1119.617208145
GTATTAG34250.017.4848521
GTCCTAG41750.017.3262461
GTCCTAA36750.016.7794271
GCCCTAG41100.015.7248251
GTACTAA33100.015.5844831
GTATAAG37950.015.1551791
GCTCTAG47800.015.0092361
GTACTAG20150.015.0070831
TACGCGG11851.0337633E-414.899145145
GTTCTAG50400.014.82317351
GTTCTAA49650.014.4499811
TTACGCG33700.014.407296145
GTGCTAG42450.014.38668251
TCTAGAC38400.014.3583013
GTATTAA54300.014.1953031
GGTCTAG34450.013.9410971
CTATACT36600.013.9305634
Produced by FastQC (version 0.11.5)