FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10419580
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA74400.047.65235145
GTACTAG19950.019.5868341
GGGGGGG518800.017.767677145
GTATTAG32850.016.0404971
GTACTAA32800.015.5234351
GTTCTAG49750.015.4708251
GTATTAA51650.015.3602291
GTGCTAG39450.014.2573821
GTCCTAG43700.014.22561551
TCTATAC36000.014.1436413
GTTCTAA49700.014.0568761
CTATACT38350.013.894554
GTATAAG34450.013.7487741
GTCCTAC38850.013.7155961
GTATAGG26800.013.6968311
TCTAGAC37450.013.5960243
GTCCTAA35250.013.4367451
CTAGACC33700.013.176464
GCCTTAG40950.012.8676521
CTAGACT37450.012.8056194
Produced by FastQC (version 0.11.5)