FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326721
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA77050.040.394775145
GTACTAG19550.018.7821461
CGCGTAT6100.00903150717.716402145
GTCCTAG44550.017.01621
GTACTAA31650.016.4668031
GTATTAG36500.016.3881071
GGGGGGG552500.015.921994145
GCGAATA15753.9143197E-715.095499145
GTGCTAG40300.014.8428271
GTCTTAG37300.014.7663931
GGTCTAG32450.014.4182551
GTATTAA52650.013.9483741
GTTCTAG46450.013.897641
GTATTAT51850.013.8209191
CTAGACT36950.013.7850224
GGTCTAA32600.013.6252341
CGAATAG12702.2232895E-413.615124145
GTTCTAA48300.013.48794651
GTGCATA41250.013.3523981
GTACTAT31600.013.3067371
Produced by FastQC (version 0.11.5)