FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326721
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG33950.024.841684145
CTGTCTA77150.024.105642145
GTCCTAG42100.018.3310241
CGACGAA15701.0786607E-917.906054145
ACGCGTT7252.1938333E-517.896555145
GTACTAG20200.017.339081
CCGTATA9202.988977E-416.453808145
GTATTAG37600.016.2620331
GTCTTAG42550.015.9048921
GTTCTAG49800.015.735121
TTACGCG45600.015.175441145
CGCCGTA23650.014.630024145
TACGCGG13351.6370905E-1114.578654145
GTGCTAG40950.014.496781
GTATAGG28550.014.1393111
GTCCTAT35850.014.0752211
GTCCTAA37400.013.8093451
GTACATA48250.013.7799031
TCTAGAC35350.013.7703723
CTAGACT35450.013.7315284
Produced by FastQC (version 0.11.5)