FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10818686
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT10000.035.507416145
CGCCGTT17450.024.916025145
GTCCTAG45550.022.1602021
GTCCTAA41700.019.6783941
GTACTAG24750.019.6583421
GTATTAG40250.019.3047921
GTTCTAG52100.018.8166941
GTGCTAG47000.018.0773721
GACGATA25150.017.863916145
GTCTTAG42350.017.8331151
GCCCTAG44800.017.8304351
GTATTAA61900.017.4800591
TTATACC70250.017.123192145
GTACAGG81850.016.9457951
GTATATA70800.016.9238031
CCGTATA13100.016.594835145
GTATACG8800.016.5042061
GTACTAA35750.016.2502941
CTAGGCC56800.016.2076054
GTCTTAA52950.016.1831591
Produced by FastQC (version 0.11.5)