FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10768942
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG39250.021.79851
GTACTAG22800.020.3530431
GTCCTAG45450.020.101151
GTATTAA59800.018.5513081
GGTCTAA36750.017.7569921
GTATATA67650.017.577631
GACGTAT20700.017.513046145
GTTCTAG50150.017.2052211
GTCCTAA39300.016.9738181
CCTATAC31850.016.844033
GTATATG58700.016.7990631
GTGCTAG47050.016.4895341
GTCTTAG44600.016.419911
CGTCTTG48800.016.343119145
CTATACT41250.016.169094
GTTCTAA55000.016.083531
GTACAGA81750.016.0536981
GTGCTAA44950.015.9694421
TGCCGTG61450.015.810477145
TATACTG58500.015.6149445
Produced by FastQC (version 0.11.5)