FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209079
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT19000.027.091825145
ACGCGTT13600.026.121052145
GTCCTAG44150.021.6856541
GTACTAG20500.020.8750461
GTACTAA34650.019.6767731
GACGATA29250.019.580992145
GTATTAG36100.019.087351
GTGCTAG41850.018.5446051
GTCTTAG40500.017.730031
GTCCTAA38400.016.8107871
CGACGAA27500.016.60892145
GGTCTAA32850.016.5598031
GTCTTAA49100.016.5449621
GTATAGG29500.016.4733541
GTTCTAG47800.016.2362291
GTTCTAA52300.015.3939681
GCTCTAG53300.015.2412331
GCCTTAG44850.015.2017881
GTATTAA60450.015.1183191
CTAGGGT35650.015.0470894
Produced by FastQC (version 0.11.5)