FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10329067
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21850.021.2396641
CGTCTTG58000.019.375242145
GTCCTAG42200.019.2452981
GTATTAG37550.018.3456361
TCTAGAC40200.018.2153133
GTCTTAG41350.018.0626241
TGCCGTG67500.018.044668145
GTTCTAG52700.017.7499941
GTATTAA58450.017.6166321
GTGCTAG42250.017.3345991
GTACTAA33650.016.5929931
GTATATA67100.016.5343841
GCTCTAG50250.016.0179161
GGGGGGG3076100.015.685235145
GTACAGA78800.015.459741
GTACATG70950.015.22835351
CTAGGAC40750.015.1228143
GTATAGA40650.014.9843481
CTATACT38650.014.8189284
GTATTGG46700.014.7511481
Produced by FastQC (version 0.11.5)