FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10329067
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT12550.033.50248145
CGCCGTT17700.024.983267145
GTCCTAG39800.021.3175051
CCGTATA17050.020.833601145
GTACTAA32850.020.0881351
GTACTAG22300.019.5110191
GTGCTAG42700.018.8507521
GTCTTAG41550.018.499861
GTATTAG37650.018.4901241
GACGATA30150.018.273382145
GTTCTAG51250.017.8283141
GTGCTAA43650.017.609831
CGACGAA27600.017.5978145
GTATAGG28900.017.3134981
GTACGGA11900.017.0625761
GTCCTAT37500.016.8237021
GTATTAA55800.016.6344851
GGTCTAA34600.016.3475281
GTACAGG80050.016.0341361
GTCTTAA47700.015.6585821
Produced by FastQC (version 0.11.5)