FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7787906
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT12150.027.441189145
GTCCTAG32000.024.0685231
CCGTATA13050.023.327068145
GTATTAG23350.021.4711721
GTACTAA24450.020.2080141
TTATACC57800.019.311422145
GTGCTAA30200.019.0070061
GTTCTAG35850.018.6462841
GTATAGG21000.018.3379251
GTCCTAA27250.018.1315941
GTGCTAG28450.017.6222111
GTCTTAG28900.017.5992361
TAGACAG47900.017.5556285
GTCCTAT26100.017.2601591
TCTAGAC25000.017.1217883
GTACAGG55250.017.09641
CCCTTAG34200.016.996421
CTAGACT24800.016.9587144
GGTCTAA23350.016.8035261
CTGCCGC143250.016.747683145
Produced by FastQC (version 0.11.5)