FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7694234
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT12450.031.433357145
CCGTATA12400.023.962303145
GTTCTAG34850.023.5573921
GTACTAG14800.021.599441
GTCCTAG30600.021.3684311
GTACTAA24200.020.4147991
GTCCTAA25750.020.3145311
GTGCTAG28350.020.2453631
CCTATAC21400.018.980753
TTATACC63600.018.459686145
GTATTAG24850.018.4189851
TCTAGAC26550.017.7577513
GTCCTAT26400.017.6127681
GTCTTAG26200.017.4699151
CCTCTAG49200.017.4248261
TAGACAG43750.017.0624925
GTATTAA37750.016.936251
GTATATA44300.016.8921551
CCCTTAG33650.016.8407381
GTATTAC25550.016.7769361
Produced by FastQC (version 0.11.5)