FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7521614
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT16400.031.82679145
CTATACG5200.023.7021274
GTACTAG15100.023.5453221
CCGTATA18350.023.308813145
GTCCTAG28600.022.8329471
GTGCTAG28250.020.8042491
GTTCTAG34600.019.2928871
GTATTAG25900.018.7698381
GCTCTAG33100.018.4135191
GTACTAA23150.018.1786861
GTATTAA39700.017.3627531
GTCCTAA24250.017.0548761
GTCTAGG28850.016.5990641
GTCTTAA34100.016.5968511
CTAGACA33450.016.4724884
TCTATAC27150.016.2898163
GTCCTAT27200.016.2722051
GTACAGG55350.016.2550961
GTTCTAA35600.016.101361
GTCCTAC26500.015.8806261
Produced by FastQC (version 0.11.5)