FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12707485
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14000.016.6585831
GGTCTAA20750.015.8675651
GTATTAG22100.014.8982811
GTCCTAG30050.014.3808071
GTTCTAG33150.014.0705981
GTCCTAA23550.013.6897061
GTATTAA30600.013.3377541
GTACTAA20950.013.26044751
GTATAAG23300.012.6590091
GTATAGG18250.012.4033291
GTATATA35950.012.4022841
CTATACT24800.012.1699324
GTATAGA24000.012.1468831
GGTCTAG22300.011.996291
GCCCTAG30300.011.8851271
GTACAGG56900.011.8743861
GTGCTAG29700.011.7787961
GTTCTAA32150.011.7346161
GTATTGG26900.011.7298571
TCTATAC25200.011.704563
Produced by FastQC (version 0.11.5)