FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12707485
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14400.020.3067321
GTCCTAG31700.014.7592161
GTACTAA21050.014.7086911
GTGCTAG28950.014.6163911
CTAGACC24800.014.1166654
GTTCTAG31600.013.9349861
GTATTAG20100.013.8634911
GTTCTAA33650.013.8016921
GTCCTAA23950.013.7895271
GTCCTAT24700.013.7886521
GGTCTAA21300.013.7284961
GCGTTAG4801.8189894E-1213.61745551
GTCTTAG27350.013.2073481
GCCCTAG30700.012.7746811
GTATTAA31100.012.7209951
GTACATA27950.012.6776581
GTCTTAA29750.012.3731561
GTATAAG24050.012.3017531
GTGTTAG28400.012.2345111
CCTCTAG51050.012.1973771
Produced by FastQC (version 0.11.5)