FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12789546
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21500.016.430791
GTACTAA20750.015.3717941
GTACTAG14750.015.346581
GTATTAA29900.014.567741
GTCCTAG29100.014.1432091
GTATATG32550.013.5924711
GTATAGG18900.013.0656321
CTAGACT25800.013.0259694
GTCTTAG25900.012.9773491
GTATATA34150.012.8552041
GTTCTAA32400.012.8085521
GTATTAT33450.012.6115571
GGTCTAA21850.012.4003911
GTTCTAG35700.012.3931361
CGATCGT5106.184564E-1112.10553838
GGTCTAG22750.012.0605831
TCTAGAC25900.011.9164373
ATAGTAC21900.011.7441293
GTCTATG34550.011.7136881
GTCTAGA25400.011.4774371
Produced by FastQC (version 0.11.5)