FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12132621
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29800.018.5884551
GTATTAG23250.018.4978261
GTACTAG14500.017.7961861
GTGCTAG29150.016.7603631
GTCCTAA23800.015.1790991
GTCTTAG25150.015.0483311
GTATTAA32700.014.9408141
GGTCTAA21850.014.8016481
GTCTTAA28450.014.1493741
GTACTAA21300.014.0531381
GTGTTAG28800.013.9774221
GTATATA35150.013.31211951
GTGCTAA27750.013.2664421
GCGATCG5750.013.1639137
GTTCTAG34600.012.8276551
CTGTACT54500.012.8153774
GGTCTAG22100.012.7660121
GTACTAT20500.012.7553791
GTCTATA21900.012.7254921
TCTAGAC25650.012.6087073
Produced by FastQC (version 0.11.5)