FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12132621
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA20650.018.3682751
GTATTAG22750.018.1884441
GTCCTAG29600.018.1730841
GTACTAG12950.017.30771
GTGCTAG30450.015.2877021
GTTCTAA31650.015.2528161
GGTCTAG21750.014.10998251
GTATAGG17600.014.10637951
GGTCTAA21500.013.9532871
CTAGACT23100.013.8523674
GTCCTAA26400.013.845151
GTTCTAG33750.013.3842251
GTATTAT31300.013.2200361
GCTCTAG37600.013.1142651
GTCTTAG25000.013.1032591
TCTAGAC25600.013.0432363
GTATATA36600.013.0015081
CTATACT27100.012.8234574
GTACATA27350.012.7338511
GCGCTAG4902.7284841E-1112.6669531
Produced by FastQC (version 0.11.5)