FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12023476
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23500.016.4297641
GTGCTAG31600.015.8183831
GTTCTAG30650.015.6338331
GTACTAT20700.015.4879441
GTACTAG13150.015.4670471
GTCCTAG30100.015.4613851
GTACTAA22650.015.0677411
GTATTAA33400.014.2434031
CGAATCG5150.014.0274389
GCTCTAG34000.013.8906581
GTATATA38650.013.8249441
GCCCTAG28850.013.7414681
GTCTTAG25600.013.6007411
GTATAGG19800.013.5803381
GTATAGA24250.013.50495151
GTGTTAG29650.013.3707051
GTCCTAA22550.013.1471941
GTTCTAA34400.013.1278631
GCTCTAA32050.012.90726951
GTATAAA42900.012.8571251
Produced by FastQC (version 0.11.5)