FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11920670
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG28850.018.4821361
GTGCTAG30600.015.2891651
GTATTAA31250.014.8610691
TATACTG31950.014.5354735
GTTCTAG35050.014.5257981
GTATTAG21200.014.441771
GTATAGG20300.014.2347381
GTACTAA21100.013.858071
ATTAGAC22050.013.7289893
GTATAAA41400.013.5442061
GTATATA37000.013.4813241
GTCTTAG25800.013.4669091
GTCCTAA25550.013.464041
GTACTAG14200.013.32418251
GCTCTAG35900.013.2236371
GTATATG33350.013.2032361
TCTGTAC52500.013.1049433
GTTCTAA32050.013.0947761
CTAGACC21900.013.0376234
GTACATA28700.012.9451821
Produced by FastQC (version 0.11.5)