FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11920670
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21450.020.3880061
GTCCTAG30550.017.471041
GTTCTAG33500.015.6241751
GTGCTAG28550.015.1971691
GTACTAG15200.014.7254331
GTATTAA31150.014.7025251
GTCCTAA24300.014.312431
TACGCGA1450.00530767914.239522
GTATAGG18500.014.1462131
GTATATA36000.014.06091
GTTCTAA33200.013.5872491
GTCCTAT26100.013.4573011
GTCTTAG26000.013.3766181
GTATTAT32250.013.2400691
GTATTGG27950.013.0593731
GTGCTAA26300.012.831241
GTATAGA25050.012.78421
GTTCTAT30500.012.6449321
GGTCTAA21200.012.5070011
GCTCTAA33150.012.4651071
Produced by FastQC (version 0.11.5)