FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11778452
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA29800.017.5443131
GTCCTAG29900.017.37061
GTATTAG21450.016.9976021
GTTCTAG29750.016.0707781
GTCTTAG25000.015.1342791
GTATAAG23000.014.9548221
GTATATA35550.014.7066251
GTACTAG13950.014.547451
TATACTG32250.014.5052015
CTATACT27250.014.3895564
CGATCGT4102.0008883E-1114.26560838
TCTATAC23150.014.2636053
GTGCTAG30050.014.1933961
GGTCTAA20700.013.9578331
CCTATAC17000.013.5561063
GTATAGG18100.013.4923891
GTATTAT29700.013.4341631
GTATAAA40700.013.3527821
GCTCTAG34900.013.1079661
GTACAGG50550.013.064391
Produced by FastQC (version 0.11.5)