FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11778452
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14450.017.390691
GTATAGG16250.016.9472161
TACGCGA2603.419882E-815.8803922
GTATACG4150.015.7604021
GTCTTAG26500.015.5882431
GTATTAG22850.015.517181
GTATTAA31650.015.1182971
GTGCTAG28150.014.5522561
GTTCTAG30050.014.54859351
GTACGCG5300.014.2892221
GTCCTAG28450.014.156811
GGTCTAG21400.013.9948191
TATACTG30750.013.9838255
GTACTAA20950.013.9667911
GTTCTAA32700.013.5801241
CTATACT23650.013.3827524
GTACAGG52350.013.283011
GTCTATA22750.013.1643551
GTACATA28600.013.1196491
GTCTTAA30450.013.1139181
Produced by FastQC (version 0.11.5)