FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14653278
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30050.016.5351121
GTACTAG19000.016.1631341
GTCCTAG36250.015.6107931
CGATCGT5350.014.83725638
GTATAAG30050.014.5830511
GCGATCG5450.014.56391937
GTACTAA29550.014.1291821
GTATTAA46600.014.0687921
GTATATA55900.013.9503721
GCTCTAG43100.013.9303211
GTCCTAA34800.013.7823941
GTGCTAG36550.013.5945311
GTCTTAG33000.013.3839751
TAGTGCG6950.013.105432557
TCTATAC31500.013.0354363
GTTCTAA41350.012.9343711
TCTAGAC34000.012.8888443
GTTCTAG40450.012.7103321
GGTCTAA27700.012.706021
CAGTACT56400.012.4807364
Produced by FastQC (version 0.11.5)