FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14697137
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17500.017.548321
GTATTAG32250.016.7978461
GTACTAA29300.016.2515621
GTCCTAG35600.016.1863961
GTATTAA44450.014.9043521
GTTCTAG40850.014.6974111
GTCTTAG31800.014.431391
GCTCTAG41750.013.8846991
GTATAGG24450.013.124651
GTATAAG28600.013.0299091
CTAGACT34200.013.0150924
CTATACT34550.012.7833764
GGTCTAA26100.012.6915421
GTACAGG65950.012.5568321
GTTCTAA41200.012.4787961
GTGCTAA37450.012.4384311
GTCTATA29050.012.2341781
GGTCTAG28500.012.2281331
GTCCTAA31050.012.2240391
GTGTACG15250.012.2182091
Produced by FastQC (version 0.11.5)